Enzymes Related to Polychlorinated Biphenyl and Enzyme Kinetics
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چکیده
منابع مشابه
Kinetics of polychlorinated biphenyl dechlorination and growth of dechlorinating microorganisms.
The present study has investigated a correlation between the kinetics of polychlorinated biphenyl (PCB) dechlorination and the growth of dechlorinating microbial populations. Microorganisms were eluted from Aroclor 1248-contaminated St. Lawrence River (NY, USA) sediments and inoculated into clean sediments spiked with Aroclor 1248 at 10 concentrations ranging from 0 to 3.12 micromol/g sediment ...
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The ability of the dehydrogenase and ring cleavage dioxygenase of the naphthalene degradation pathway to transform 3,4-dihydroxylated biphenyl metabolites was investigated. 1,2-Dihydro-1, 2-dihydroxynaphthalene dehydrogenase was expressed as a histidine-tagged protein. The purified enzyme transformed 2, 3-dihydro-2,3-dihydroxybiphenyl, 3,4-dihydro-3,4-dihydroxybiphenyl, and 3,4-dihydro-3,4-dihy...
متن کاملCharacterization of biphenyl dioxygenase of Pandoraea pnomenusa B-356 as a potent polychlorinated biphenyl-degrading enzyme.
Biphenyl dioxygenase (BPDO) catalyzes the aerobic transformation of biphenyl and various polychlorinated biphenyls (PCBs). In three different assays, BPDO(B356) from Pandoraea pnomenusa B-356 was a more potent PCB-degrading enzyme than BPDO(LB400) from Burkholderia xenovorans LB400 (75% amino acid sequence identity), transforming nine congeners in the following order of preference: 2,3',4-trich...
متن کاملIi. Soil Enzymes and Enzyme Kinetics
Suggested Readings: Tabatabai, M. A. 1982. Soil enzymes. p. 903-915, 943-947. In A. L. Page, R. H. Miller, and D. R. Keeney (eds.), Methods of Soil Analysis, Part 2. American Society of Agronomy, Madison, Wisconsin. Dick, W. A. and M. A. Tabatabai. 1992. Significance and potential uses of soil enzymes. p. 95127. In F. Blaine Metting (ed.), Soil Microbial Ecology. Marcel Dekker, New York. Ruggie...
متن کاملAerobic Polychlorinated Biphenyl-Degrading Bacteria
Several bacterial isolates were characterized based on their abilities to degrade specific polychlorinated biphenyls (PCBs) and their 16s rRNA gene sequences. The members of one group of bacteria consisting of Alcaligenes species, including the PCB-degrading bacterium Alcaligenes eutrophus H850, had strong abilities to degrade a broad range of PCBs but not the di-para-chlorine-substituted conge...
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ژورنال
عنوان ژورنال: Biochemistry & Analytical Biochemistry
سال: 2012
ISSN: 2161-1009
DOI: 10.4172/2161-1009.1000e124